CHROMATIN: A PRACTICAL APPROACH

Chromatin comprises the complex of nucleic acids and proteins that go to make up the chromosomes, and regulation of its structure is increasingly recognized as an important factor in gene expression. Researchers from related fields needing an introduction to the methodology of chromatin will find this book invaluable, as will experienced workers. 1: Isolation and fractionation of chromatin and linker histones 2: Extraction and separation of core histones and non-histone chromosomal proteins 3: Mapping chromatin structure in Drosophila 4: In vivo footprint and chromatin analysis by LMPCR 5: Analysis of nuclear scaffold attachment regions 6: Electron microscopy of chromatin 7: Counting nucleosome cores on circular DNA using topoisomerase I 8: In vitro reconstitution of chromatin and analysis of nucleosome positioning 9: Transcriptional and structural analysis of chromatin assembled in vivo 10: An assembly of chromatin and nuclear structures in Xenopus egg extracts Appendix: List of Suppliers Index Readership: Post-graduates and post-doctoral researchers, and academic staff