CIRCADIAN RHYTHMS. METHODS AND PROTOCOLS

Methods for analysis of rhythmic readouts in select model organisms Novel techniques for identifying clock genes by mutagenesis and mircroarrays Procedures for isolation, extraction, and analysis of RNA Procedures for extraction, fractionation, and purification of proteins Detailed in vitro protocols for circadian research Methods for microscopy analysis in circadian biology This is an in-depth examination of circadian biology, presented by leading researchers in the field. This cutting-edge collection of protocols is adaptable for research at every level, and represents the huge strides that chronobiologists have made over the past two decades. These robust, reproducible procedures take into account the myriad of variables that circadian researchers must be cognizant of while conducting their research. By distilling the arsenal of methodologies available, and presenting essential procedures in precise detail, this book supports a vast majority of circadian research efforts. Part I provides a concise introduction to essential components of circadian biology. Part II presents protocols and techniques for monitoring and analyzing rhythmic patterns of several model organisms. Parts III, IV, and V emphasize the identification of clock genes and protein and RNA expression in circadian biology. Protocols for in vitro systems comprise Part VI, and Part VII provides an outline of methods and tips for microscopy analysis.