A LABORATORY COURSE IN TISSUE ENGINEERING

This book fills the need for a hands-on lab manual for tissue engineering instruction. Providing more than a semester’s worth of activities, it contains fifteen standalone experiments based on both classic and modern approaches. Experiments encompass a set of widely applicable techniques: cell culture, microscopy, histology, immunohistochemistry, mechanical testing, soft lithography, and common biochemical assays. In addition to teaching these specific techniques, the experiments emphasize engineering analysis, mathematical modeling, and statistical experimental design. To minimize lab costs, experiments use equipment commonly found in labs equipped for tissue culture.